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pclncx vector  (Novus Biologicals)


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    Structured Review

    Novus Biologicals pclncx vector
    Pclncx Vector, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pclncx+retrovirus+expression+vector/pCLNCX+Retrovirus+Expression+Vector/10__1038_slash_nchembio__1578-506-15-17
    Average 90 stars, based on 6 article reviews
    pclncx vector - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Methods and compositions for detecting neoplastic cells
    Article Snippet: For polarity analysis, a customized Matlab program was used to evenly divide a HUVEC into 36 angular sections with the bead position as the centre and the force direction as the zero degree axes for v, as illustrated in FIG. 7, panel C. For statistical analysis of long-range activation of Src, a pixel located within a cell at a given time is defined as ‘distally activated’ when: (1) its emission ratio value is above a certain percentage (98%, 90%, 80% and 50% were used as the thresholds) of pixel population in the same cell after 15 min of force stimulation, and (2) the distance between the pixel and the centre of the force-imposed bead is larger than half of the virtual radius of the cell, as shown in the following formula: ( x - x 0 ) 2 + ( y - y 0 ) 2 > R 2 = 1 2 A π where x, y are the coordinates of any given pixel within the cell body, x0, y0 are the coordinates of the force-imposed bead centre, R is the virtual radius for the cell, and A is the area of the cell. .. To generate retrovirus expression plasmids, the various Src reporters were inserted into the HindIII/XhoI restriction sites of the PCLNCX retrovirus expression vector (Imgenex). ..

    Article Title: Assembly of endocytic machinery around individual influenza viruses during viral entry
    Article Snippet: The stable cell line was created using the RetroMax retroviral expression system (Imgenex). .. The EYFP-LCa gene was cloned into the Hin d III/ Cla I restriction sites of the pCLNCX retrovirus expression vector (Imgenex) that confers G418 resistance. .. 293T cells, growing in Dulbecco’s Modified Eagle’s Medium (DMEM) containing 10% (v/v) FBS, were then transfected using this vector and the pCL-10A1 retrovirus packaging vector (Imgenex).

    Plasmid Preparation:

    Article Title: Methods and compositions for detecting neoplastic cells
    Article Snippet: For polarity analysis, a customized Matlab program was used to evenly divide a HUVEC into 36 angular sections with the bead position as the centre and the force direction as the zero degree axes for v, as illustrated in FIG. 7, panel C. For statistical analysis of long-range activation of Src, a pixel located within a cell at a given time is defined as ‘distally activated’ when: (1) its emission ratio value is above a certain percentage (98%, 90%, 80% and 50% were used as the thresholds) of pixel population in the same cell after 15 min of force stimulation, and (2) the distance between the pixel and the centre of the force-imposed bead is larger than half of the virtual radius of the cell, as shown in the following formula: ( x - x 0 ) 2 + ( y - y 0 ) 2 > R 2 = 1 2 A π where x, y are the coordinates of any given pixel within the cell body, x0, y0 are the coordinates of the force-imposed bead centre, R is the virtual radius for the cell, and A is the area of the cell. .. To generate retrovirus expression plasmids, the various Src reporters were inserted into the HindIII/XhoI restriction sites of the PCLNCX retrovirus expression vector (Imgenex). ..

    Article Title: Assembly of endocytic machinery around individual influenza viruses during viral entry
    Article Snippet: The stable cell line was created using the RetroMax retroviral expression system (Imgenex). .. The EYFP-LCa gene was cloned into the Hin d III/ Cla I restriction sites of the pCLNCX retrovirus expression vector (Imgenex) that confers G418 resistance. .. 293T cells, growing in Dulbecco’s Modified Eagle’s Medium (DMEM) containing 10% (v/v) FBS, were then transfected using this vector and the pCL-10A1 retrovirus packaging vector (Imgenex).

    Clone Assay:

    Article Title: Assembly of endocytic machinery around individual influenza viruses during viral entry
    Article Snippet: The stable cell line was created using the RetroMax retroviral expression system (Imgenex). .. The EYFP-LCa gene was cloned into the Hin d III/ Cla I restriction sites of the pCLNCX retrovirus expression vector (Imgenex) that confers G418 resistance. .. 293T cells, growing in Dulbecco’s Modified Eagle’s Medium (DMEM) containing 10% (v/v) FBS, were then transfected using this vector and the pCL-10A1 retrovirus packaging vector (Imgenex).



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